Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells
作者： Lei Guo , Shumin Zhang , Bo Zhang , Wanyong Chen , Xiaoqiang Li
DOI: 10.1007/S10565-016-9327-Z
关键词: Immunology 、 Downregulation and upregulation 、 Cell growth 、 Gene silencing 、 Messenger RNA 、 Gene knockdown 、 Cancer research 、 Biology 、 Cell culture 、 Cell 、 Signal transduction
摘要: G2 and S phase-expressed-1 (GTSE1) was recently reported to upregulate in several types of human cancer, based on negatively regulate p53 expression. However, its expression functional roles hepatocellular carcinoma (HCC) remain unknown. In this study, GTSE1 observed be highly expressed HCC specimens cell lines both at messenger RNA (mRNA) protein levels. Furthermore, high positively associated with tumor size, venous invasion, advanced stage, short overall survival. Moreover, we generated stable knockdown explore the effects silencing growth invasion vitro. determining pathway through which regulated proliferation found inhibit AKT phosphorylation downregulated cycle-related protein. addition, downregulation decreased xenografts. conclusion, these results indicated for first time that overexpression involved progress HCC, enhancing promoting cells.
springer.com
core.ac.uk
paperity.org参考文章(24)
Yanxia Sui, Xiaoqiang Zheng, Dongli Zhao, Rab31 promoted hepatocellular carcinoma (HCC) progression via inhibition of cell apoptosis induced by PI3K/AKT/Bcl-2/BAX pathway Tumor Biology. ,vol. 36, pp. 8661- 8670 ,(2015) , 10.1007/S13277-015-3626-5
Vivek Mishra, Kausar M. Ansari, Raj Khanna, Mukul Das, Role of ErbB2 mediated AKT and MAPK pathway in gall bladder cell proliferation induced by argemone oil and butter yellow. Argemone oil and butter yellow induced gall bladder cell proliferation. Cell Biology and Toxicology. ,vol. 28, pp. 149- 159 ,(2012) , 10.1007/S10565-011-9207-5
Yitong Liu, Thomas J. Flynn, Menghang Xia, Paddy L. Wiesenfeld, Martine S. Ferguson, Evaluation of CYP3A4 inhibition and hepatotoxicity using DMSO-treated human hepatoma HuH-7 cells Cell Biology and Toxicology. ,vol. 31, pp. 221- 230 ,(2015) , 10.1007/S10565-015-9306-9
Yanfeng Liu, Ju-Bo Zhang, Yi Qin, Wei Wang, Lili Wei, Yigang Teng, Lei Guo, Bo Zhang, Zhenhai Lin, Jing Liu, Zheng-Gang Ren, Qing-Hai Ye, Youhua Xie, PROX1 promotes hepatocellular carcinoma metastasis by way of up‐regulating hypoxia‐inducible factor 1α expression and protein stability Hepatology. ,vol. 58, pp. 692- 705 ,(2013) , 10.1002/HEP.26398
Massimilano Scolz, Per O. Widlund, Silvano Piazza, Debora Rosa Bublik, Simone Reber, Leticia Y. Peche, Yari Ciani, Nina Hubner, Mayumi Isokane, Martin Monte, Jan Ellenberg, Anthony A. Hyman, Claudio Schneider, Alexander W. Bird, GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration PLoS ONE. ,vol. 7, pp. e51259- ,(2012) , 10.1371/JOURNAL.PONE.0051259
Mariarosaria D’Errico, Emanuele de Rinaldis, Monica F. Blasi, Valentina Viti, Mario Falchetti, Angelo Calcagnile, Francesco Sera, Calogero Saieva, Laura Ottini, Domenico Palli, Fabio Palombo, Alessandro Giuliani, Eugenia Dogliotti, Genome-wide expression profile of sporadic gastric cancers with microsatellite instability European Journal of Cancer. ,vol. 45, pp. 461- 469 ,(2009) , 10.1016/J.EJCA.2008.10.032
Martin Monte, Roberta Benetti, Licio Collavin, Luigi Marchionni, Giannino Del Sal, Claudio Schneider, hGTSE-1 expression stimulates cytoplasmic localization of p53. Journal of Biological Chemistry. ,vol. 279, pp. 11744- 11752 ,(2004) , 10.1074/JBC.M311123200
M Monte, L Collavin, D Lazarevic, R Utrera, T.A Dragani, C Schneider, Cloning, chromosome mapping and functional characterization of a human homologue of murine gtse-1 (B99) gene. Gene. ,vol. 254, pp. 229- 236 ,(2000) , 10.1016/S0378-1119(00)00260-2
Jing Ding, Qin-Ying Li, Jie-Zhong Yu, Xin Wang, Chuan-Zhen Lu, Cun-Gen Ma, Bao-Guo Xiao, The lack of CD131 and the inhibition of Neuro-2a growth by carbamylated erythropoietin. Cell Biology and Toxicology. ,vol. 31, pp. 29- 38 ,(2015) , 10.1007/S10565-015-9292-Y
X Shawn Liu, Hongchang Li, Bing Song, Xiaoqi Liu, Polo‐like kinase 1 phosphorylation of G2 and S‐phase‐expressed 1 protein is essential for p53 inactivation during G2 checkpoint recovery EMBO Reports. ,vol. 11, pp. 626- 632 ,(2010) , 10.1038/EMBOR.2010.90