Mutational and recombinational events in carcinogen-modified plasmid DNA. Influence of host-cell repair genes.
作者： Peter J. Abbott
DOI: 10.1016/0167-8817(85)90036-7
关键词: DNA 、 Repressor lexA 、 Molecular biology 、 Plasmid 、 Mutation frequency 、 Transformation (genetics) 、 AP site 、 Gene 、 Mutagenesis 、 Biology
摘要: Abstract A plasmid containing the STR operon has been modified in vitro (i) by irradiation with UV light, (ii) reaction ethyl methanesulphonate (EMS), (iii) N-acetoxy-2-acetylaminofluorene (AcO-AAF), (iv) (±)trans-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), and (v) heating at 70°C to produce apurinic sites. Suitably DNA was then used transform both repair-proficient repair-deficient strains of Escherichia coli, mutation frequency plasmid-encoded rspL+ gene measured. The influence host mutations uvrB+, recA+, umuC+ lexA+, genes on have investigated. Transformation into a uvrB strain significantly decreased survival increased level observed for UV- AcO-AAF-modified DNA, while only small increase seen EMS-modified no Mutagenesis BPDE-modified (and probably also sites) totally dependent recA+ product, EMS AcO-AAF induced mutagenesis partially independent gene. or umuC lexA strain, other hand, showed change from that wild-type strain. Pre-irradiation light before transformation led significant DNA. These results are discussed terms mutational recombinational pathways which may be available act suggest majority events measured this system due recombination between homologous regions chromosomal
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