作者: A. Pinson
DOI: 10.1007/978-3-642-75262-9_2
关键词: Beating heart 、 Cardiac muscle cell 、 Proteolytic enzymes 、 Biology 、 Embryonic stem cell 、 Cell culture 、 Neonatal rat 、 Myocyte 、 Cell biology 、 Embryonic chick
摘要: Tremendous progress has been made since the pioneering, but long overlooked, observation of Burrows [5] in 1912 that single beating heart cells migrated away from embryonic chick isolated tissue expiants. suggested his finding supported myogenic theory for cardiac activity. Several decades passed before realization systems, such as culture, might serve aas useful tools studying biological systems began to take hold. For this reason, although tryptic dissociation, reported by Rous and Jones [68] 1916, Carrel’s method subculturing [6] have available many years, science had wait until 1950s these techniques be revived Moscona [56], who tissues proteolytic digestion. He also determined which essential materials, cofactors, vitamins were required successfully maintain cell cultures.