Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity
作者： Piya Paul , Punit Bansal , Pawan G. Nayak , Steve Thomas Pannakal , K.I. Priyadarsini
DOI: 10.1016/J.ETAP.2011.09.007
关键词: Genotoxicity 、 Organic chemistry 、 DNA damage 、 Pharmacology 、 Rutin 、 Polyphenol 、 Lipid peroxidation 、 Cytotoxicity 、 Quercetin 、 Chemistry 、 TBARS
摘要: Abstract Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction Pilea microphylla (PM1) with that its active constituents against γ-radiation in V79 cells. PM1 standardized respect polyphenols present by RP-HPLC. It evaluated for free radical scavenging potential using Fenton reaction-induced DNA damage lipid peroxidation. Further, subjected γ-radiation-induced cytotoxicity genotoxicity significantly reduced radical-mediated calf thymus Among concentrations tested (12.5, 25 50 μg/ml) radioprotection, at 25 μg/ml exhibited maximum protection. when compared constituent viz., rutin, quercetin chlorogenic acid (concentrations equivalent PM1-25 μg/ml), a combination found most effective preventing genotoxicity. To conclude, radioprotection is possibly synergistic effect phytochemicals herbal extract, rather than any single component.
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