Cellular force microscopy for in vivo measurements of plant tissue mechanics.

摘要: Although growth and morphogenesis are controlled by genetics, physical shape change in plant tissue results from a balance between cell wall loosening intracellular pressure. Despite recent work demonstrating role for mechanical signals morphogenesis, precise measurement of properties at the individual level remains technical challenge. To address this challenge, we have developed cellular force microscopy (CFM), which combines versatility classical microindentation techniques with high automation resolution approaching that atomic microscopy. CFM’s large range forces provides possibility to map apparent stiffness both plasmolyzed turgid as well perform micropuncture cells using very stresses. CFM experiments reveal that, within tissue, local measurements can vary turgor pressure an unexpected way. Altogether, our highlight importance detailed physically based simulations interpretation results. ability be used assess manipulate mechanics makes it method choice unravel feedbacks mechanics, morphogenesis.