Exploration du potentiel thérapeutique des cellules souches embryonnaires humaines pour la thérapie cellulaire de la maladie de Huntington
作者： Laetitia Aubry
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摘要: La maladie de Huntington (MH) est une neurodegenerative rare, qui affecte les neurones GABAergiques moyens epineux (MSN) du striatum. Actuellement aucun traitement ne permet guerir cette pathologie. Un essai clinique pilote, fonde sur la greffe intracerebrale tissus fœtaux humains, a permis remplacer cellules lesees et corriger certains symptomes. Neanmoins, logistique necessaire pour acceder ces limite forme therapie cellulaire un nombre patients restreint. Il d’identifier source alternative capables efficacement fœtaux. Les souches embryonnaires humaines (hES) possedent deux proprietes essentielles, l’autorenouvellement pluripotence, en font des candidats interessants. L’objectif notre etude ete d’evaluer le potentiel therapeutique MH. Nous avons elabore protocole permettant production, in vitro, progeniteurs striataux se differencier MSN, partir hES. Une fois greffes dans striatum lese rat, peuvent survivre MSN. Ces experiences xenogreffes ont d’autres parts reveles presence neurales proliferatives persistantes. L’ensemble nos resultats demontre que hES constituent pertinente Cependant, ils soulignent necessite mettre place mesures specifiques controler proliferation vivo greffons issus hES, avant d’envisager toute application clinique.
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