The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.

作者: Xue-Chao Hu , Lu-Jing Ren , Sheng-Lan Chen , Li Zhang , Xiao-Jun Ji

DOI: 10.1007/S00449-015-1452-1

关键词: Yield (chemistry)FermentationIndustrial and production engineeringSchizochytrium sp.Docosahexaenoic acidCell growthBiologyOsmotic pressureDry weightChromatographyFood science

摘要: The effects of different osmotic pressure, changed by six salts (NaCl, Na2SO4, (NH4)2SO4, KH2PO4 and MSG), on cell growth DHA synthesis Schizochytrium sp. were investigated. Six optimal mediums obtained to study pressure combinations at stage stage. Results showed that cultivated in higher condition fermented lower was benefit enhance synthesis. Combination 17-6 could get the maximum dry weight 56.95 g/L highest percentage total fatty acids 55.21 %, while combination 17-B lipid yield 33.47 with 42.10 % acids. This first report about enhancement production regulation this work provided two novel control processes for high

参考文章(29)
Yohtalou TASHIMA, Nobuko YOSHIMURA, Control of Rabbit Liver Fructose-1, 6-diphosphatase Activity by Magnesium Ions Journal of Biochemistry. ,vol. 78, pp. 1161- 1169 ,(1975) , 10.1093/OXFORDJOURNALS.JBCHEM.A131012
Jing Li, Ruijie Liu, Guifang Chang, Xiangyu Li, Ming Chang, Yuanfa Liu, Qingzhe Jin, Xingguo Wang, None, A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources. Bioresource Technology. ,vol. 177, pp. 51- 57 ,(2015) , 10.1016/J.BIORTECH.2014.11.046
Lu-Jing Ren, Li-Na Sun, Xiao-Yan Zhuang, Liang Qu, Xiao-Jun Ji, He Huang, Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition Bioprocess and Biosystems Engineering. ,vol. 37, pp. 865- 872 ,(2014) , 10.1007/S00449-013-1057-5
Lu-Jing Ren, Guan-nan Sun, Xiao-Jun Ji, Xue-chao Hu, He Huang, Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp. Bioresource Technology. ,vol. 157, pp. 107- 113 ,(2014) , 10.1016/J.BIORTECH.2014.01.078
Mutsumi Takagi, Karseno, Toshiomi Yoshida, Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. Journal of Bioscience and Bioengineering. ,vol. 101, pp. 223- 226 ,(2006) , 10.1263/JBB.101.223
Xiangyu Li, Ye Lin, Ming Chang, Qingzhe Jin, Xingguo Wang, Efficient production of arachidonic acid by Mortierella alpina through integrating fed-batch culture with a two-stage pH control strategy. Bioresource Technology. ,vol. 181, pp. 275- 282 ,(2015) , 10.1016/J.BIORTECH.2015.01.009
D. Le Rudulier, A. R. Strom, A. M. Dandekar, L. T. Smith, R. C. Valentine, Molecular Biology of Osmoregulation Science. ,vol. 224, pp. 1064- 1068 ,(1984) , 10.1126/SCIENCE.224.4653.1064
Kyeong Ho Min, Hwan Hee Lee, Periasamy Anbu, Bidur Prasad Chaulagain, Byung Ki Hur, The effects of culture condition on the growth property and docosahexaenoic acid production from Thraustochytrium aureum ATCC 34304 Korean Journal of Chemical Engineering. ,vol. 29, pp. 1211- 1215 ,(2012) , 10.1007/S11814-011-0287-Y
Li-Bo Yang, Xiao-Bei Zhan, Zhi-Yong Zheng, Jian-Rong Wu, Min-Jie Gao, Chi-Chung Lin, A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol. Bioresource Technology. ,vol. 151, pp. 120- 127 ,(2014) , 10.1016/J.BIORTECH.2013.10.031
Lu-Jing Ren, Xiao-Jun Ji, He Huang, Liang Qu, Yun Feng, Qian-Qian Tong, Ping-Kai Ouyang, Development of a stepwise aeration control strategy for efficient docosahexaenoic acid production by Schizochytrium sp. Applied Microbiology and Biotechnology. ,vol. 87, pp. 1649- 1656 ,(2010) , 10.1007/S00253-010-2639-7