Molecular cloning and nucleotide sequence of the gramicidin S synthetase 1 gene.

作者: Kazuko Hori , Yoshihiro Yamamoto , Toshitaka Minetoki , Toshitsugu Kurotsu , Masayuki Kanda

DOI: 10.1093/OXFORDJOURNALS.JBCHEM.A122909

关键词: BiochemistryGramicidinMolecular cloningTyrocidineAmino acidMolecular biologyPeptide sequenceNucleic acid sequenceGene productBiologyGene

摘要: The entire gene for gramicidin S synthetase 1 (GS 1) was cloned into the plasmid vector pUC18, and nucleotide sequences of GS its flanking region were determined. full-length clone 4,539 base pairs long had an open reading frame 3,294 nucleotides coding 1,098 amino acids. calculated molecular weight 123,474 agreed with apparent 120,000 found in SDS-PAGE from B. brevis. sequence highly homologous to that tyrocidine 1. overall similarity between deduced acid two genes 57.5%. product GS309 easily purified essentially homogeneous state by ammonium sulfate fractionation followed DEAE-Sepharose CL-6B, Ultrogel AcA-34, second CL-6B column chromatography. protein catalyzed D-phenylalanine-dependent ATP-32PPi exchange reaction which is specific activity, activity nearly same as also showed a weak phenylalanine racemase activity.

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