Alignment of direct detection device micrographs using a robust Optical Flow approach.

作者: Vahid Abrishami , Javier Vargas , Xueming Li , Yifan Cheng , Roberto Marabini

DOI: 10.1016/J.JSB.2015.02.001

关键词: Image (mathematics)Computer visionArtificial intelligenceMotion correctionProteasome endopeptidase complexSoftwareOptical flowArchaeal ProteinsComputer scienceCorrection method

摘要: The introduction of direct detection devices in cryo-EM has shown that specimens present beam-induced motion (BIM). Consequently, in this work, we develop a BIM correction method …

参考文章(23)
H. Shigematsu, F.J. Sigworth, Noise models and cryo-EM drift correction with a direct-electron camera. Ultramicroscopy. ,vol. 131, pp. 61- 69 ,(2013) , 10.1016/J.ULTRAMIC.2013.04.001
Christopher R. Booth, Wen Jiang, Matthew L. Baker, Z. Hong Zhou, Steven J. Ludtke, Wah Chiu, A 9 Å single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope Journal of Structural Biology. ,vol. 147, pp. 116- 127 ,(2004) , 10.1016/J.JSB.2004.02.004
Axel F. Brilot, James Z. Chen, Anchi Cheng, Junhua Pan, Stephen C. Harrison, Clinton S. Potter, Bridget Carragher, Richard Henderson, Nikolaus Grigorieff, Beam-induced motion of vitrified specimen on holey carbon film. Journal of Structural Biology. ,vol. 177, pp. 630- 637 ,(2012) , 10.1016/J.JSB.2012.02.003
J. Vargas, R. Restrepo, T. Belenguer, Shack - Hartmann spot dislocation map determination using an optical flow method Optics Express. ,vol. 22, pp. 1319- 1329 ,(2014) , 10.1364/OE.22.001319
Melody G. Campbell, Anchi Cheng, Axel F. Brilot, Arne Moeller, Dmitry Lyumkis, David Veesler, Junhua Pan, Stephen C. Harrison, Clinton S. Potter, Bridget Carragher, Nikolaus Grigorieff, Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy Structure. ,vol. 20, pp. 1823- 1828 ,(2012) , 10.1016/J.STR.2012.08.026
Robert M. Glaeser, Richard J. Hall, Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects Biophysical Journal. ,vol. 100, pp. 2331- 2337 ,(2011) , 10.1016/J.BPJ.2011.04.018
Anna-Clare Milazzo, Philippe Leblanc, Fred Duttweiler, Liang Jin, James C. Bouwer, Steve Peltier, Mark Ellisman, Fred Bieser, Howard S. Matis, Howard Wieman, Peter Denes, Stuart Kleinfelder, Nguyen-Huu Xuong, Active pixel sensor array as a detector for electron microscopy. Ultramicroscopy. ,vol. 104, pp. 152- 159 ,(2005) , 10.1016/J.ULTRAMIC.2005.03.006