A novel steroid and cytotoxic constituents from Dioscorea membranacea Pierre against hepatocellular carcinoma and cholangiocarcinoma cells

摘要: Abstract Ethnopharmacological relevance The rhizomes of Dioscorea membrancea Pierre have been used in Thai traditional medicine as an ingredient formula for liver cancer and cholangiocarcinoma treatment. Aim the study To investigate cytotoxic activity ethanolic extract constituents D. to support its use. Materials methods SRB assay was determine against hepatocellular carcinoma (HepG2), (KKU-M156) cells one normal human keratinocyte immortal (HaCaT) with isolated compounds. Bioassay guided isolation isolating Results D . membranacea rhizome showed weak KKU-M156 HepG2 (IC 50 at 72 h exposure=30.49±0.82 38.97±2.04 µg/mL respectively). A new steroid [epipanthogenin B ( 1 )], a known [panthogenin 2 two napthofuranoxepins [dioscorealide 3 ) dioscorealide 4 phenanthraquinone [dioscoreanone 5 )] phenanthrene [5,6-dihydroxy-2,4-dimethoxy-9,10-dihydrophenanthrene 6 2,5,6-trihydroxy-3,4-dimethoxy, 9, 10-dihydrophenanthrene 7 were from active chloroform fraction. Compound highest cytotoxicity exposure=2.87±0.21 µM) exposure=1.67±0.10 µM) less toxicity cell line exposure>100 µM). selective exposure=3.46±0.11 µM). Compounds exposure=24.96±2.32 51.31±3.52 µM). – no lines 72 h Conclusion Seven compounds fraction rhizomes. Only might be served good anticancer agent because it can kill but not toxic on cell. This research use