Noncoding 3' sequences of the transferrin receptor gene are required for mRNA regulation by iron

摘要: The cell-surface receptor for transferrin mediates cellular uptake of iron from serum. Transferrin protein and mRNA levels are increased in cells treated with chelating agents, decreased by treatment salts or hemin. Here we report that expression human cDNA constructions stably transfected mouse fibroblasts is regulated both the chelator, desferrioxamine, We found sequences within 3' noncoding region required iron-dependent feed-back regulation expression, whereas presence promoter not necessary. Regulation observed when transcription initiated at either SV-40 early promoter, but deletion a 2.3 kb fragment 2.6 abolishes increases constitutive level expression. Furthermore, does affect decrease receptors which response to growth arrest, indicating controlled least two distinct mechanisms.