Control of albumin and α-fetoprotein expression in rat liver and in some transplantable hepatocellular carcinomas

作者: Stewart Sell , Klewyn Thomas , Michael Michalson , Jose Salatrepat , James Bonner

DOI: 10.1016/0005-2787(79)90198-9

关键词: Amino acidRadioimmunoassayCellImmunofluorescenceMolecular biologyAlbuminRNAMessenger RNABiologyComplementary DNABiochemistry, Genetics and Molecular Biology (miscellaneous)

摘要: Abstract Albumin and α-fetoprotein production by rat liver four selected transplantable hepatocellular carcinomas is compared to the messenger RNA present in these tissues. were measured radioimmunoassay of serum concentration, immunofluorescence, vitro incorporation labeled amino acids into proteins specifically precipitated antisera. The number mRNA molecules per cell was calculated from hybridization specific cDNA probes polysomal translational activity a wheat germ system. amount albumin produced different tissues directly related functional for each protein.

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