Autofluorescence of adipose tissue measured with fibre optics.

摘要: The optical absorbance and fluorescence of porcine subcutaneous adipose tissue were measured directly with fibre optics. Either mercury or xenon light sources used to illuminate samples through one branch a bifurcated guide. Fluorescence was detected the other Grating, prism continuous interference filter monochromators installed at appropriate locations in path measurements made computer-operated photomultiplier whose dynamic range optimized each wavelength measured. There small peak 420 nm. With excitation 365 nm, emission spectrum 510 nm secondary plateau from 430 450 Proline powder purified biochemical Type III collagen also autofluorescent Adipose cells associated bovine heart valves examined by microscopy obtain preparations containing plus all three histological types connective fibres on same section. Reticular (around cells) together elastin (in valves) autofluorescent. It is suggested that major source reticular surround cells, minor contributions such as collagenous septa, vascular and, possibly, cytoplasmic components.