Manganese peroxidase isoenzymes produced by Pleurotus ostreatus grown on wood sawdust.
作者： Paola Giardina , Gianna Palmieri , Bianca Fontanella , Vincenzo Rivieccio , Giovanni Sannia
关键词: Pleurotus ostreatus 、 Enzyme 、 Biochemistry 、 Peroxidase 、 Molecular cloning 、 Manganese peroxidase 、 Versatile peroxidase 、 Isozyme 、 Chemistry 、 Sawdust
摘要: Abstract The white rot basidiomycete Pleurotus ostreatus produces two manganese peroxidase (MnP) isoenzymes when grown in solid stationary conditions on poplar sawdust, whereas a lower production of these same enzymes is observed fir sawdust. Addition Mn 2+ to culture resulted threefold increase MnP activity; the addition was able tenfold production. (MnP2 and MnP3) were purified from P. culture. differ their p I values, molecular masses, N-terminal sequences. MnP3 has sequence as that previously reported. Both exhibit -dependent -independent activities tested phenolic substrates. gene coding for new isoenzyme MnP2 cloned sequenced promoter region analyzed. Furthermore, chromosomal localization all known genes determined.
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Ute Raeder, Paul Broda, Preparation and characterization of DNA from lignin-degrading fungi Methods in Enzymology. ,vol. 161, pp. 211- 220 ,(1988) , 10.1016/0076-6879(88)61022-6
M. G. Paice, I. D. Reid, R. Bourbonnais, F. S. Archibald, L. Jurasek, Manganese Peroxidase, Produced by Trametes versicolor during Pulp Bleaching, Demethylates and Delignifies Kraft Pulp. Applied and Environmental Microbiology. ,vol. 59, pp. 260- 265 ,(1993) , 10.1128/AEM.59.1.260-265.1993
T.L. Poulos, S.L. Edwards, H. Wariishi, M.H. Gold, Crystallographic refinement of lignin peroxidase at 2 A. Journal of Biological Chemistry. ,vol. 268, pp. 4429- 4440 ,(1993) , 10.1016/S0021-9258(18)53627-9
Robert Bourbonnais, Michael G. Paice, Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Letters. ,vol. 267, pp. 99- 102 ,(1990) , 10.1016/0014-5793(90)80298-W
G. Palmeiri, P. Giardina, L. Marzullo, B. Desiderio, G. Nittii, R. Cannio, G. Sannia, Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus. Applied Microbiology and Biotechnology. ,vol. 39, pp. 632- 636 ,(1993) , 10.1007/BF00205066
Sovan Sarkar, Angel T Martı́nez, Marı́a Jesús Martı́nez, Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus Biochimica et Biophysica Acta. ,vol. 1339, pp. 23- 30 ,(1997) , 10.1016/S0167-4838(96)00201-4
Shi Yu Fu, Hui-sheng Yu, John A Buswell, Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor‐caju Fems Microbiology Letters. ,vol. 147, pp. 133- 137 ,(1997) , 10.1111/J.1574-6968.1997.TB10232.X
Yasuhiko Asada, Akira Watanabe, Toshikazu Irie, Toru Nakayama, Masaaki Kuwahara, Structures of genomic and complementary DNAs coding for Pleurotus ostreatus manganese (II) peroxidase. Biochimica et Biophysica Acta. ,vol. 1251, pp. 205- 209 ,(1995) , 10.1016/0167-4838(95)00102-Z
Gianna Palmieri, Paola Giardina, Carmen Bianco, Andrea Scaloni, Antonio Capasso, Giovanni Sannia, A Novel White Laccase from Pleurotus ostreatus Journal of Biological Chemistry. ,vol. 272, pp. 31301- 31307 ,(1997) , 10.1074/JBC.272.50.31301
Tünde Mester, Jim A. Field, Characterization of a Novel Manganese Peroxidase-Lignin Peroxidase Hybrid Isozyme Produced by Bjerkandera Species Strain BOS55 in the Absence of Manganese Journal of Biological Chemistry. ,vol. 273, pp. 15412- 15417 ,(1998) , 10.1074/JBC.273.25.15412