Cobra venom proteome and glycome determined from individual snakes of Naja atra reveal medically important dynamic range and systematic geographic variation.

摘要: Abstract Recent progress in snake venomics has shed much light on the intra-species variation among toxins from different geographical regions and provided important information for better snakebite management. Most previous reports were based venoms pooled snakes. In this study, we present proteomic glycomic profiles of individual Naja atra The results reveal wide dynamic range three-finger toxins. Systematic classification cardiotoxin (CTX-) A2/A4 A6, respectively, allowed identification two putative subspecies Taiwan cobra eastern western regions. We also identified four major N-glycan moieties venom metalloproteinase bi-antennary glycan core. ELISA showed that these glycoproteins ( Biological significance Based obtained snakes, demonstrated a surprisingly venomics. This provides reasonable explanation variable neutralization effects antivenom treatment victims suffering suggests simple economic method to produce potent with efficacy. Since venomic distinct ranges observed isolated regions, current profile should be used as quality control future production clinical applications.