Amperometric l-lactate-sensing electrode based on a polyion complex layer containing lactate oxidase. Application to serum and milk samples
作者： Fumio Mizutani , Soichi Yabuki , Yoshiki Hirata
DOI: 10.1016/0003-2670(95)00278-8
关键词: Enzyme electrode 、 Ion selective electrode 、 Lactic acid 、 Semipermeable membrane 、 Aqueous solution 、 Amperometry 、 Ascorbic acid 、 Chemistry 、 Detection limit 、 Chromatography
摘要: Abstract An amperometric enzyme electrode for l -lactic acid was prepared by immobilizing lactate oxidase (LOD) in a polyion complex membrane. aqueous solution containing poly- -lysine and LOD placed on glassy carbon electrode, an of poly(4-styrenesulfonate) added to the polycation/LOD mixture dried. The anodic current (at 1 V vs. Ag AgCl ) this increased immediately after addition L response time observed up 0.3 mM with detection limit 0.1 μM. applied determination sera sour milk: membrane showed permselectivity based solute size molecular weight cut-off ca. 100, which very effective suppressing electrochemical interference -ascorbic acid, uric acetaminophen. could be used at least two months.
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