N-(1-pyrenyl) maleimide induces bak oligomerization and mitochondrial dysfunction in Jurkat Cells.
作者： Pei-Rong Huang , Shu-Chen Hung , Chia-Chu Pao , Tzu-Chien V. Wang
DOI: 10.1155/2015/798489
关键词: Bcl-2 Homologous Antagonist-Killer Protein 、 Bcl-2-associated X protein 、 Jurkat cells 、 Caspase 8 、 Molecular biology 、 Biology 、 Mitochondrion 、 Cytosol 、 Apoptosis 、 Cell biology 、 Cytochrome c
摘要: N-(1-pyrenyl) maleimide (NPM) is a fluorescent reagent that frequently used as derivatization agent for the detection of thio-containing compounds. NPM has been shown to display great differential cytotoxicity against hematopoietic cancer cells. In this study, molecular mechanism by which induces apoptosis was examined. Here, we show treatment Jurkat cells with leads Bak oligomerization, loss mitochondrial membrane potential (Δψm), and release cytochrome C from mitochondria cytosol. Induction oligomerization appears play critical role in NPM-induced apoptosis, downregulation shRNA significantly prevented apoptosis. Inhibition caspase 8 Z-IETD-FMK and/or depletion Bid did not affect Bak. Taken together, these results suggest mediated through pathway independent caspase-8 activation.
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