Viable, but non-culturable, state of a green fluorescence protein-tagged environmental isolate of Salmonella typhi in groundwater and pond water
作者： Jang-Cheon Cho , Sang-Jong Kim
DOI: 10.1111/J.1574-6968.1999.TB13382.X
关键词: Viable Cell Count 、 Microbiology 、 Bone plate 、 Enterobacteriaceae 、 Aequorea victoria 、 Biology 、 Bacteria 、 Green fluorescent protein 、 Escherichia coli 、 Salmonella typhi
摘要: An environmental isolate of Salmonella typhi was chromosomally marked with a gfp gene encoding green fluorescence protein (GFP) isolated from Aequorea victoria. The hybrid transposon mini-Tn5 transconjugated E. coli to S. typhi, resulting in constitutive GFP production. survival GFP155 introduced into groundwater and pond water microcosms examined by GFP-based plate counts, total cell direct viable counts. A comparison between counts good method for verifying the viable, but non-culturable (VBNC), state typhi. entry VBNC shown all microcosms. survived longer than as both culturable state.
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