Structure of the provirus within NIH 3T3 cells transfected with Harvey sarcoma virus DNA.

摘要: Abstract NIH 3T3 cells transformed with unintegrated Harvey sarcoma virus (HSV) linear DNA generally acquired a complete HSV provirus. Infection of these Moloney murine leukemia helper was followed by release infectious particles. The provirus within transfected convalently joined to nonviral sequences and termed "cell-linked" DNA. association this cell-virus sequence the chromosomal cell unclear. NIH could also become transfection cell-linked In case, recipient donor fragment containing both its flanking sequences. After either or DNA, newly established cellular underwent amplifications between 5 100 copies per diploid cell. may acquire deleted proviral lacking at least 1.3 kilobase pairs from right end full-length 6-kilobase-pair (corresponding 3'-proximal portion wild-type RNA). Cells bearing such genomes were transformed, indicating that viral transformation gene lies in middle 5'-proximal RNA genome. However, when infected virus, only low levels biologically active particles released. Therefore, 3' required for efficient transmission