6 Yeast Hybrid Approaches

摘要: Publisher Summary This chapter discusses the exploitation of yeast genetics to create artificial selection/reporter systems suitable for yielding insights into signaling function any organism. The two-hybrid (Y2H) system was designed in late 1980s as a means detecting direct interactions between binary pairs proteins. There were two compelling reasons develop such technology. One facilitate analysis physical requirements interaction defined proteins already known or suspected interact. second allow identification novel partners interest, thereby obtaining clues biological these Among advantages using purposes low cost and speed assays involving this organism availability large number well-characterized effective expression reporter systems. Many different Y2H approaches have been described chapter. Some used effectively by many researchers. Others one initial reports, but not followed up extensively.