Multi-enhancer transcriptional hubs confer phenotypic robustness.
作者： Albert Tsai , Mariana RP Alves , Justin Crocker
DOI: 10.7554/ELIFE.45325
关键词: Drosophila melanogaster 、 Transcription factor 、 Cell biology 、 Transcriptional regulation 、 Transcription (biology) 、 Locus (genetics) 、 Gene expression 、 Biology 、 Enhancer 、 Ultrabithorax
摘要: We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and active svb on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis these multi-enhancer 'hubs' improve phenotypic resilience stress by buffering against decreases transcription factor concentrations output. Deleting a redundant enhancer from locus led reduced trichome numbers raised at elevated temperatures. Using high-resolution fluorescence microscopy, observed lower Ubx concentration output this deletion allele. Transcription sites of full cis-regulatory region inserted into chromosome colocalized with locus, increasing concentration, svb, partially rescuing phenotype. Thus, multiple could reinforce local hub buffer environmental stresses genetic perturbations, providing mechanism for phenotypical robustness.
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