Nanoinitiator for enzymatic anaerobic polymerization and graft enhancement of gelatin-PAAM hydrogel.

摘要: As an emerging method for mildly molding polymer hydrogel bioscaffolds, the enzymatically polymerized system is mainly based on screening of various oxidoreductases to produce radicals, but design multifunctional nanoinitiators facilitate performance remains challenging. Here, we utilize N-hydroxyimide-modified silica nanoparticles as simultaneously trigger glucose oxidase anaerobic polymerization and nanoparticle-grafting enhancement gelatin–polyacrylamide (PAAM) hydrogel. The enzyme–nanoinitiator produced nitrogen which were further converted into carbon radicals via GOx-catalyzed reduction, confirmed by electron paramagnetic resonance (EPR) analysis. Our stretchable has a 12-fold increased fracture energy relative traditional due grafting nanoinitiator. temperature-dependent physical crosslinking gelatin endowed our facile printing ability. Cytotoxicity assay 3D cell culture demonstrated low toxicity first example use enzymatic polymerization, this work provides biocompatible platform prepare or print bioscaffolds with required mechanical strength.