Strategic tillage increased the relative abundance of Acidobacteria but did not impact on overall soil microbial properties of a 19-year no-till Solonetz

摘要: Continuous no-till (NT) farming is widely practiced in Australia, but it prone to weed infestation. Strategic tillage (ST) can be used effectively control weeds; however, unclear whether ST influences soil microbial properties. We investigated one- or two-time events using a chisel plough influence the properties of an acidic Solonetz with 19-year NT management. Soil samples were collected from 0–10 and 10–20 cm depths, 1 year post-ST after chickpea crop. Microbial biomass C (MBC) N (MBN), community-level physiological profiling (CLPP, MicroResp™) fluorescein diacetate as indicator total activity (TMA) determined soil. The composition communities was profiled terminal reaction fragment length polymorphism (T-RFLP) 16S rRNA sequencing. Detection DNA-based quantitation ChitinaseA, nifH, amoA, narG, nirK nosZ genes assess effects on cycling. Our results show that did not change MBC/MBN, TMA CLPP. Likewise, abundance encoding enzymes involved key steps reactions at either depth. However, one-time increased relative Acidobacteria RB41 iii1-15, slightly average utilisation, both This suggests even cropping season chickpea, negligible. One- could potentially address issues associated long-term without impacting overall