Characterization of the effect of retinol on Plasmodium falciparum in vitro

摘要: A preliminary study from our laboratory found retinol (vitamin alcohol) to have in vitro activity against Plasmodium falciparum at concentrations close those normal human serum (1-3 muM). To characterize the antimalarial potential of more detail, 3D7 and K1 strains P. were maintained continuous culture [H-3]hypoxanthine incorporation microscopy used assess effect asexual stages parasite life-cycle. Losses retinol-associated hemolysis also quantified system. There losses >50% but no was observed with added up 100 M. All development showed comparable sensitivity including merozoite invasion (range mean IC50 values 10.1-21.4 muM after adjustment for losses). Retinol pre-treatment uninfected RBC did not inhibit invasion. treatment associated increased vacuolization within food vacuole evidence membrane rupture. These appearances similar seen quinoline artemisinin compounds. Although these data do support a role acute supplementation malaria, they add knowledge regarding therapies justify assessment potent synthetic retinoids their metabolites. (C) 2004 Elsevier Inc. rights reserved.