Identification of the Herboxidiene Biosynthetic Gene Cluster in Streptomyces chromofuscus ATCC 49982
作者： Lei Shao , Jiachen Zi , Jia Zeng , Jixun Zhan
DOI: 10.1128/AEM.06904-11
关键词: DNA 、 Genetics 、 Biochemistry 、 Gene 、 DNA sequencing 、 Gene cluster 、 Gene Knockout Techniques 、 Streptomyces 、 Sequence analysis 、 Biology 、 Herboxidiene
摘要: ABSTRACTThe 53-kb biosynthetic gene cluster for the novel anticholesterol natural product herboxidiene was identified inStreptomyces chromofuscusATCC 49982 by genome sequencing and gene inactivation. In addition to herboxidiene, a biosynthetic intermediate, 18-deoxy-herboxidiene, was also isolated from the fermentation broth ofS. chromofuscusATCC 49982 as a minor metabolite.
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