High-Resolution Imaging with 99mTc-Glucarate for Assessing Myocardial Injury in Rat Heart Models Exposed to Different Durations of Ischemia with Reperfusion

摘要: In mammals, D-glucaric acid (glucarate, GLA) is a 6-carbon dicarboxylic sugar and natural catabolite of glucuronic metabolism (1,2). All mammals excrete glucaric as physiologic end product. GLA can be readily radiolabeled with sodium pertechnetate, resulting in 99mTc-GLA. Experimental studies have shown the favorable targeting potential 99mTc-GLA severe ischemia or early necrosis heart brain (1-4). acutely injured cells, it believed that associated disruption myocyte nuclear membranes, allowing free intracellular diffusion electrochemical binding negatively charged complex to positively histones. This distribution driven by avidity protein cytoplasmic proteins positive charge. rat models isoproterenol-induced myocardial infarction, uptake infarcted hearts was found 6 times more than normal rats (5). has been proposed specific oncotic marker very stages injury but not an apoptotic (6). As infarct-avid agent, necrotic myocardium rapid blood-pool clearance suggest important diagnostic detection acute infarction identification successful revascularization therapy. To date, few done regarding imaging ischemic—reperfused varied severity injury. study conducted determine whether amount damage ischemia—reperfusion accumulation levels It known this association reflected degree hearts. First, estimated size infarct irreversible myocytic necrosis. clinical patients, determined several noninvasive techniques, which significance for general prognosis patients after attack. Since accumulated only, we speculated hot spot might ideal noninvasively determining SPECT camera. Using well-defined vivo models, acquired images novel high-resolution stationary system called FASTSPECT. Ischemia—reperfusion analyzed triphenyltetrazolium chloride (TTC) staining electron microscope examination. We quantified compared TTC analysis. Second, since washout kinetics viability-dependent agents are altered extent cellular extracellular structural changes myocardium, present undertaken kinetic properties assessing exposed different durations left coronary artery (LCA) occlusion reperfusion. FASTSPECT, dynamic tomographic were obtained beating no motion detectors aperture. wanted clarify sequence 3-dimensional (3D) FASTSPECT make possible visualize investigate cardiovascular small-animal models.