PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells
作者： Ee-Chun Cheng , Dongwan Kang , Zhong Wang , Haifan Lin
DOI: 10.1371/JOURNAL.PONE.0097821
关键词: Reprogramming 、 Cell biology 、 Cellular differentiation 、 Stem cell 、 Embryonic stem cell 、 Piwi-interacting RNA 、 SOX2 、 Genetics 、 Induced pluripotent stem cell 、 Biology 、 Homeobox protein NANOG 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: PIWI proteins play essential and conserved roles in germline development, including stem cell maintenance meiosis. Because regulators such as OCT4, NANOG, SOX2 are known to be potent factors that reprogram differentiated somatic cells into induced pluripotent (iPSCs), we investigated whether the protein family is involved iPSC production. We find all three mouse Piwi genes, Miwi, Mili, Miwi2, expressed embryonic (ESCs) at higher levels than fibroblasts, with Mili being highest. However, mice lacking genes viable female fertile, only male sterile. Furthermore, fibroblasts derived from Miwi/Mili/Miwi2 triple knockout embryos can efficiently reprogrammed iPS cells. These pluripotency markers were capable of differentiating germ layers teratoma assays. Genome-wide expression profiling reveals very similar littermate control results indicate dispensable for direct reprogramming fibroblasts.
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