Tat Mediates Apoptosis in Vivo in the Rat Central Nervous System

作者: Eleftherios S. Gavriil , Ryan Cooney , Benjamin S. Weeks

DOI: 10.1006/BBRC.1999.1894

关键词: StriatumIn vivoTUNEL assayBiologyMolecular biologyIn Situ Nick-End LabelingApoptosisCentral nervous systemCell biologyTransactivationDNA fragmentation

摘要: The HIV-1 transactivator of transcription, Tat, mediates apoptosis in neurons and may cause AIDS-associated neurologic disorders, which include dementia loss motor control. Here we investigate the ability Tat to stimulate rat central nervous system vivo. Using TUNEL assay, treatment pheochromocytoma (PC12) cells with 1.0 microgram/ml for 24 h was found apoptosis. Further, electrophoresis DNA from Tat-treated PC12 demonstrated fragmentation. To mediated vivo, 20 microgram infused into striatum Sprague-Dawley rats. Histochemical analysis (TUNEL) Tat-infused area these rats postural deviation ipsilateral infusion. These data demonstrate that stimulates vivo causes dysfunction intact animal.

参考文章(12)
B.S. Weeks, K. Desai, P.M. Loewenstein, M.E. Klotman, P.E. Klotman, M. Green, H.K. Kleinman, Identification of a novel cell attachment domain in the HIV-1 Tat protein and its 90-kDa cell surface binding protein. Journal of Biological Chemistry. ,vol. 268, pp. 5279- 5284 ,(1993) , 10.1016/S0021-9258(18)53530-4
Gary Wormser, None, AIDS and other manifestations of HIV infection : Lippincott-Raven. ,(2004)
J M Sabatier, E Vives, K Mabrouk, A Benjouad, H Rochat, A Duval, B Hue, E Bahraoui, Evidence for neurotoxic activity of tat from human immunodeficiency virus type 1. Journal of Virology. ,vol. 65, pp. 961- 967 ,(1991) , 10.1128/JVI.65.2.961-967.1991
Dale E. Bredesen, Robert M. Levy, M. L. Rosenblum, AIDS And the Nervous System ,(1988)
Deborah R. New, Sanjay B. Maggirwar, Leon G. Epstein, Stephen Dewhurst, Harris A. Gelbard, HIV-1 Tat Induces Neuronal Death via Tumor Necrosis Factor-α and Activation of Non-N-methyl-d-aspartate Receptors by a NFκB-Independent Mechanism Journal of Biological Chemistry. ,vol. 273, pp. 17852- 17858 ,(1998) , 10.1074/JBC.273.28.17852
J Cheng, A Nath, B Knudsen, S Hochman, J.D Geiger, M Ma, D.S.K Magnuson, Neuronal excitatory properties of human immunodeficiency virus type 1 tat protein Neuroscience. ,vol. 82, pp. 97- 106 ,(1997) , 10.1016/S0306-4522(97)00174-7
B. S. Weeks, D. M. Lieberman, B. Johnson, E. Roque, M. Green, P. Loewenstein, E. H. Oldfield, H. K. Kleinman, NEUROTOXICITY OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 TAT TRANSACTIVATOR TO PC12 CELLS REQUIRES THE TAT AMINO ACID 49-58 BASIC DOMAIN Journal of Neuroscience Research. ,vol. 42, pp. 34- 40 ,(1995) , 10.1002/JNR.490420105