Dissection of abscisic acid signal transduction pathways in barley aleurone layers.

作者: Qingxi Shen , Aurelio Gomez-Cadenas , Pengnian Zhang , Mary Kay Walker-Simmons , Jen Sheen

DOI: 10.1023/A:1011667312754

关键词: Gene expressionSignal transductionGibberellinAleuroneGene productGeneAbscisic acidBiochemistryABI1Biology

摘要: Abscisic acid (ABA) induces genes that are highly expressed during late embryogenesis, but suppresses gibberellin (GA)-responsive essential for seed germination and seedling growth. Promoter elements necessary sufficient ABA up- down-regulation of gene expression have been previously defined in barley aleurone layers. We studied the effect a protein phosphatase 2C, ABI1, an ABA-inducible kinase, PKABA1, transcription factor, VP1, on action transient system. The observations allowed us to dissect signal transduction pathways leading either induction or suppression expression. embryogenesis is inhibited presence mutated encoded by abi1-1 dominant mutant known block responses Arabidopsis. However, product has no GA-responsive α-amylase gene. On other hand, PKABA1 genes, little up-regulated genes. Therefore, it appears follow two separate with former ABI1 latter modulated PKABA1. VP1 enhances also specific A schematic model based these presented explain regulatory proteins ABA-mediated

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