Methods of plant root exudates analysis: a review
作者： Peter Dundek , Ladislav Holík , Tomáš Rohlík , Ladislav Hromádko , Valerie Vranová
DOI: 10.11118/ACTAUN201159030241
关键词: Gas chromatography–mass spectrometry 、 Polysaccharide 、 Freeze-drying 、 Total organic carbon 、 Ultrafiltration 、 Chromatography 、 Rhizosphere 、 Gel electrophoresis of proteins 、 Amino acid 、 Chemistry
摘要: DUNDEK, P., HOLIK, L., ROHLIK, T., VRANOVA, V., REJSEK, K., FORMANEK, P.: Methods of plant root exudates analysis: a review. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 3, pp. 241–246 The aim this review is to summarise current knowledge on methods being used determine individual compounds and properties water-soluble exudates. These include amino acids, organic acids simple sugars, as well polysaccharides, proteins substances. Qualitative composition exudation rate are commonly measured with the consequent synthetic preparation be supplied soil create artifi cial rhizosphere for diff erent experimental purposes. Root collection usually requires fi ltration or centrifugation remove solids, detritus microbial cell debris, concentration using an evaporator, lyophilizator ultrafi ltration. analysis total groups (total carbohydrates) carbon simple. On other hand, HPLC GS/MS analyse low molecular weight molecules (sugars, acids) separation columns. Other such pH, conductivity activity enzymes gel electrophoresis sometimes assessed. All these discussed in work.
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