Detection of Wild-Type Hypoxanthine Guanine Phosphoribosyl Transferase of Lymphocytes in Gamma-Irradiated Mice with Surface Plasmon Resonance
作者： Xiaohong Zhang , Zhichao Lou , Ailian Wang , Haiqian Zhang
DOI: 10.1080/00032719.2012.655677
关键词: Detection limit 、 Hypoxanthine 、 Mutant 、 Guanine 、 Hypoxanthine-guanine phosphoribosyltransferase 、 Biosensor 、 Surface plasmon resonance 、 Biochemistry 、 Wild type 、 Molecular biology 、 Chemistry
摘要: A quick and sensitive detection of the wild-type hypoxanthine guanine phosphoribosyl transferase (HPRT), which is known as a biomarker for radiation exposure, was developed. The conventional HPRT measurement technique to detect mutant HPRT, time-consuming has low sensitivity. In this study, detected gamma using surface plasmon resonance (SPR) biosensor with 6-thioguanine (6-TG) probe anti-HPRT antibody signal amplification factor. First, we used system measure dissolved in PBS. Six-TG immobilized on can specifically enhances about 10 times primary produced by binding 6-TG. linear relationship (r = 0.991) obtained between concentration enhanced signal. limit (LDL) 2.1 ng/mL. regeneration glycine-HCl also investiga...
sci-hub.se 参考文章(33)
Erika Kress-Rogers, Handbook of Biosensors and Electronic Noses: Medicine, Food, and the Environment ,(1996)
Sally A Amundson, Michael Bittner, Paul Meltzer, Jeffrey Trent, Albert J Fornance, Jr, Biological indicators for the identification of ionizing radiation exposure in humans. Expert Review of Molecular Diagnostics. ,vol. 1, pp. 211- 219 ,(2001) , 10.1586/14737159.1.2.211
Giordana Feriotto, Roberto Corradini, Stefano Sforza, Nicoletta Bianchi, Carlo Mischiati, Rosangela Marchelli, Roberto Gambari, Peptide nucleic acids and biosensor technology for real-time detection of the cystic fibrosis W1282X mutation by surface plasmon resonance. Laboratory Investigation. ,vol. 81, pp. 1415- 1427 ,(2001) , 10.1038/LABINVEST.3780355
Chi-Chun Fong, Wan-Ping Lai, Yun-Chung Leung, Samuel C.-L. Lo, Man-Sau Wong, Mengsu Yang, Study of substrate-enzyme interaction between immobilized pyridoxamine and recombinant porcine pyridoxal kinase using surface plasmon resonance biosensor. Biochimica et Biophysica Acta. ,vol. 1596, pp. 95- 107 ,(2002) , 10.1016/S0167-4838(02)00208-X
Dimitris Kyprianou, Antonio R. Guerreiro, Martin Nirschl, Iva Chianella, Sreenath Subrahmanyam, Anthony P.F. Turner, Sergey Piletsky, The application of polythiol molecules for protein immobilisation on sensor surfaces Biosensors and Bioelectronics. ,vol. 25, pp. 1049- 1055 ,(2010) , 10.1016/J.BIOS.2009.09.030
Hao Tang, Qiong Wang, Qingji Xie, Youyu Zhang, Liang Tan, Shouzhuo Yao, Enzymatically biocatalytic precipitates amplified antibody-antigen interaction for super low level immunoassay: an investigation combined surface plasmon resonance with electrochemistry. Biosensors and Bioelectronics. ,vol. 23, pp. 668- 674 ,(2007) , 10.1016/J.BIOS.2007.08.001
R.J. Albertini, L.M. Sullivan, J.K. Berman, C.J. Greene, J.A. Stewart, J.M. Silveira, J.P. O'Neill, Mutagenicity monitoring in humans by autoradiographic assay for mutant T lymphocytes. Mutation Research\/genetic Toxicology. ,vol. 204, pp. 481- 492 ,(1988) , 10.1016/0165-1218(88)90043-2
Renjun Pei, Xiurong Yang, Erkang Wang, Enhanced surface plasmon resonance immunoassay for human complement factor 4 Analytica Chimica Acta. ,vol. 453, pp. 173- 179 ,(2002) , 10.1016/S0003-2670(01)01417-9
Yongcan Wang, Xu Zhu, Minghua Wu, Ning Xia, Jianxiu Wang, Feimeng Zhou, Simultaneous and label-free determination of wild-type and mutant p53 at a single surface plasmon resonance chip preimmobilized with consensus DNA and monoclonal antibody. Analytical Chemistry. ,vol. 81, pp. 8441- 8446 ,(2009) , 10.1021/AC9014269
Esa Stenberg, Björn Persson, Håkan Roos, Csaba Urbaniczky, Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins Journal of Colloid and Interface Science. ,vol. 143, pp. 513- 526 ,(1991) , 10.1016/0021-9797(91)90284-F