THE INTERCELLULAR TRANSFER OF MOLECULES IN TISSUE CULTURE CELLS: A KINETIC STUDY BY MULTICHANNEL MICROFLUOROMETRY

摘要: ABSTRACT: In situ kinetic studies on the intercellular transfer of molecules can be achieved by multisite microfluorometry tracer or intracellular fluorochromes, in combination with microinjection tracers (e.g. fluorescein, 6:carboxy fluorescein isothiocyanate glutamic) metabolites glucose-6-P). Other fluorescence photographic scanning techniques are available to study but sensitivity and temporal resolution multichannel required determine kinetics flux (or their catabolites) detected via NAD(P) ⇄ NAD(P)H transients. The transit time τ has been evaluated a variety cell cultures (liver, glia, cultivated pancreatic islet cells, fibroblasts) where is confirmed visual observation. these varied from 0.4 sec 1 sec. No highly malignant ascites (EL2) melanoma (HPM) cultures. “Communicating territories” defined clusters liver as seen spread preferentially into some secondary tertiary cells adjacent injected tracer, while there no other cells. glucose-6-P one its observable NCTC 8739 spontaneously transformed L grown mitochondrial inhibitor atractylate. preparations islets, which show extensive within cluster 10–15 cells), reverse transients (i.e. instead NAD(P)H) observed. Using suitable biological α, β δ exhibiting different endocrine activities) intra metabolic interactions may studied whole functional system.