Comprehensive exploration of novel chimeric transcripts in clear cell renal cell carcinomas using whole transcriptome analysis
作者： Masahiro Gotoh , Hitoshi Ichikawa , Eri Arai , Suenori Chiku , Hiromi Sakamoto
DOI: 10.1002/GCC.22211
关键词: Sanger sequencing 、 FHIT 、 Gene 、 Fusion gene 、 Biology 、 Transcriptome 、 Reverse transcriptase 、 Molecular biology 、 Gene expression profiling 、 Clear cell
摘要: The aim of this study was to clarify the participation expression chimeric transcripts in renal carcinogenesis. Whole transcriptome analysis (RNA sequencing) and exploration candidate using deFuse program were performed on 68 specimens cancerous tissue (T) 11 non-cancerous cortex (N) obtained from patients with clear cell carcinomas (RCCs) an initial cohort. As positive controls, two RCCs associated Xp11.2 translocation analyzed. After verification by reverse transcription (RT)-PCR Sanger sequencing, 26 novel identified 17 (25%) RCCs. Genomic breakpoints determined five transcripts. Quantitative RT-PCR revealed that mRNA levels for MMACHC, PTER, EPC2, ATXN7, FHIT, KIFAP3, CPEB1, MINPP1, TEX264, FAM107A, UPF3A, CDC16, MCCC1, CPSF3, ASAP2 genes, being partner genes involved cohort, significantly reduced T samples relative corresponding N second Moreover, above correlated tumor aggressiveness poorer patient outcome, indicating these may participate malignant progression is case when their are reduced, also not fully function These data suggest generation carcinogenesis inducing dysfunction tumor-related genes.
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