Identification of 6-deoxy-d-talose in an extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain X6C61

摘要: Abstract An extracellular polysaccharide made by the obligately anaerobic ruminal bacterium Butyrivibrio fibrisolvens was purified anion-exchange chromatography and found to contain an unknown carbohydrate component. This component homogeneity preparative paper column on Dowex 50W-X4-immobilized calcium ions. Reaction in cysteine-sulfuric acid test suggested that a 6-deoxyhexose, supposition confirmed electron impact chemical ionization mass spectrometric studies of alditol acetate derivatives prepared from sugar. On basis results obtained analytical with three different solvent systems, tentatively identified as 6-deoxytalose. The being 6-deoxy- d -talose g.l.c. analysis acetylated glycosides it chiral (−)-2-octanol. These were compared corresponding sets diastereometric - l -talose.