Rotational Dynamics of Biological Macromolecules
作者： Edward Blatt , Thomas M. Jovin
DOI: 10.1007/978-94-009-4726-9_15
关键词: Phosphorescence 、 Triplet state 、 Molecular physics 、 Rotational correlation time 、 Anisotropy 、 Rotational diffusion 、 Chemistry 、 Analytical chemistry 、 Fluorescence 、 GSD microscopy 、 Excitation
摘要: The rotational diffusion of biological macromolecules is very sensitive to shape, size, and environmental constraints. Large scale global motions occur in the μ-ms time domain, as case intrinsic membrane proteins. We describe methods for measuring motion free, liganded, cell-surface associated states. They are based on long-lived photophysical processes detection a polarized emission process: phosphorescence, delayed fluorescence, ground state depletion (monitored by fluorescence), fluorescence from transient intermediates. In every case, signals derived different combinations excitation polarization states used construct time-resolved (anisotropy) function addition primary decay curve. Thus, lifetimes correlation times can be determined, generally multi-component exponential analysis.
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