Fetal Hemoglobin-Containing Cells Have the Same Mean Corpuscular Hemoglobin as Cells Without Fetal Hemoglobin: A Reciprocal Relationship Between Gamma- And Beta-Globin Gene Expression in Normal Subjects and in Those With High Fetal Hemoglobin Production

摘要: We have developed methodology that allows comparison of the mean corpuscular hemoglobin (MCH) fetal (HbF)-containing red cells (F cells) with MCH non-F from same individual. To do this, suspensions peripheral blood erythrocytes and their internal contents are fixed an imidodiester, dimethyl-3,3′- dithiobispropionimidate dihydrochloride (DTBP). Thereafter made permeable to antisera by treatment Triton X-100 isopropanol, reacted a mouse monoclonal antibody (MoAb) against HbF, then fluorescein-conjugated antimouse IgG. No appreciable is lost during such manipulation. Red diversity subjects were thus treated examined microscopically, first transmitted light epifluorescence. A direct correlation between Coulter-derived absorbance 415 nm was found for 100 unfixed (r = 0.96) antibody-treated fixed-permeabilized 0.99) among individuals selected so as provide range Coulter values 20 35. Comparisons microscopically derived F statistically nondistinguishable (P greater than 0.05) in all subjects. Such comparisons included normal (less 1% cells), SS patients (7% 48% congenital anemia (22% 65% heterocellular hereditary persistence HbF (HPFH) (12% 21% heterozygotes beta + thalassemia (11% 31% cells). conclude gamma- beta-globin production within regulated reciprocal fashion both elevated production.