Cascade immunoassay by multiple binding reactions
作者： James P. Mapes , Randal A. Hoke
DOI:
关键词: Substrate (chemistry) 、 Analyte 、 Immunoassay 、 Vesicle 、 Ligand (biochemistry) 、 Enzyme 、 Lysis 、 Chromatography 、 Chemistry 、 Conjugated system
摘要: A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, antianalyte affixed to solid support and tracer having conjugated thereto. bound fraction is separated from the incubated in second with masked ligand. The ligand converted by on give free lignad which binds antiligand. signal system, such as substrate therefor, or label-loaded vesicle lysing agent, added generate used detect measure analyte liquid. invention kit materials useful performing assay invention.
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