Gene expression profiling for seed protein and oil synthesis during early seed development in soybean

作者: Young Eun Jang , Moon Young Kim , Sangrea Shim , Jayern Lee , Suk-Ha Lee

DOI: 10.1007/S13258-015-0269-2

关键词: ArabidopsisBiologyBiotin carboxyl carrier proteinGene expression profilingProtein biosynthesisGeneticsBiochemistryGene familyGeneGene expressionAmino acid permeaseMolecular biology

摘要: Soybean (Glycine max L.) is one of the most important crops because its high seed contents protein and oil. However, little known about molecular regulation filling during accumulation oil storage products. We identified soybean homologs Arabidopsis genes involved in metabolic pathways carbon precursors, protein, oil, analyzed gene expression patterns immature seeds at 1 2 weeks after flowering (WAF). G. undergoes two rounds whole-genome duplication; number three synthesis more than times higher that Arabidopsis. Among these genes, five were conserved as single-copy 44 copy families consisting seven homolog members. differentially expressed aged between WAF, including CELL WALL INVERTASE, BRANCHED-CHAIN AMINO ACID TRANSAMINASE, PERMEASE, ALDEHYDE REDUCTASE, BIOTIN CARBOXYL CARRIER PROTEIN. Expression analysis duplicated on synteny block revealed had stronger positive correlations their those genes. This study provides novel insights into details associated with pathways. These can be used tools to improve nutrient composition.

参考文章(56)
Charlie Scrimgeour, Chemistry of Fatty Acids Bailey's Industrial Oil and Fat Products. pp. 1- 40 ,(2005) , 10.1002/047167849X.BIO005
Dmitry A. Los, Norio Murata, Structure and expression of fatty acid desaturases Biochimica et Biophysica Acta. ,vol. 1394, pp. 3- 15 ,(1998) , 10.1016/S0005-2760(98)00091-5
J. Chung, H. L. Babka, G. L. Graef, P. E. Staswick, D. J. Lee, P. B. Cregan, R. C. Shoemaker, J. E. Specht, The Seed Protein, Oil, and Yield QTL on Soybean Linkage Group I Crop Science. ,vol. 43, pp. 1053- 1067 ,(2003) , 10.2135/CROPSCI2003.1053
Haiwang Li, Tuanjie Zhao, Yufeng Wang, Deyue Yu, Shouyi Chen, Ruibao Zhou, Junyi Gai, Genetic structure composed of additive QTL, epistatic QTL pairs and collective unmapped minor QTL conferring oil content and fatty acid components of soybeans Euphytica. ,vol. 182, pp. 117- 132 ,(2011) , 10.1007/S10681-011-0524-9
E. C. Brummer, G. L. Graef, J. Orf, J. R. Wilcox, R. C. Shoemaker, Mapping QTL for Seed Protein and Oil Content in Eight Soybean Populations Crop Science. ,vol. 37, pp. 370- 378 ,(1997) , 10.2135/CROPSCI1997.0011183X003700020011X
S. H. Lee, M. A. Bailey, M. A. R. Mian, T. E. Carter, E. R. Shipe, D. A. Ashley, W. A. Parrott, R. S. Hussey, H. R. Boerma, RFLP loci associated with soybean seed protein and oil content across populations and locations. Theoretical and Applied Genetics. ,vol. 93, pp. 649- 657 ,(1996) , 10.1007/BF00224058
Pawan Khera, Hari D. Upadhyaya, Manish K. Pandey, Manish Roorkiwal, Manda Sriswathi, Pasupuleti Janila, Yufang Guo, Michael R. McKain, Ervin D. Nagy, Steven J. Knapp, James Leebens‐Mack, Joann A. Conner, Peggy Ozias‐Akins, Rajeev K. Varshney, Single Nucleotide Polymorphism-based Genetic Diversity in the Reference Set of Peanut (Arachis spp.) by Developing and Applying Cost-Effective Kompetitive Allele Specific Polymerase Chain Reaction Genotyping Assays The Plant Genome. ,vol. 6, pp. 1- 11 ,(2013) , 10.3835/PLANTGENOME2013.06.0019
Zhanglin Cui, Thomas E. Carter, Joseph W. Burton, Randy Wells, Phenotypic Diversity of Modern Chinese and North American Soybean Cultivars Crop Science. ,vol. 41, pp. 1954- 1967 ,(2001) , 10.2135/CROPSCI2001.1954
Eleni Bachlava, Ralph E. Dewey, Joseph W. Burton, Andrea J. Cardinal, Mapping and Comparison of Quantitative Trait Loci for Oleic Acid Seed Content in Two Segregating Soybean Populations Crop Science. ,vol. 49, pp. 433- 442 ,(2009) , 10.2135/CROPSCI2008.06.0324
Yi Zhang, Licong Yi, Yongjun Lin, Lili Zhang, Zongze Shao, Ziduo Liu, Characterization and site-directed mutagenesis of a novel class II 5-enopyruvylshikimate-3-phosphate (EPSP) synthase from the deep-sea bacterium Alcanivorax sp. L27. Enzyme and Microbial Technology. ,vol. 63, pp. 64- 70 ,(2014) , 10.1016/J.ENZMICTEC.2014.02.010