Proteomic analysis of hematopoietic stem cell-like fractions in leukemic disorders.
作者： Jun Ota , Yoshihiro Yamashita , Katsuya Okawa , Hiroyuki Kisanuki , Shin-ichiro Fujiwara
关键词: Heat shock protein 、 Molecular biology 、 Biology 、 Stem cell 、 Proteomics 、 Genetics 、 Nuclear protein 、 Carcinogenesis 、 Proteome 、 Transcriptome 、 Hematopoietic stem cell
摘要: DNA microarray analysis has been applied to identify molecular markers of human hematological malignancies. However, the relatively low correlation between abundance a given mRNA and that encoded protein makes it important characterize profile directly, or 'proteome,' malignant cells in addition 'transcriptome.' To proteins specifically expressed leukemias, here we isolated AC133(+) hematopoietic stem cell-like fractions from bone marrow 13 individuals with various leukemic disorders, compared their profiles by two-dimensional electrophoresis. A total 11 differentially spots corresponding 10 independent were detected, peptide fingerprinting combined mass spectrometry these revealed them include NuMA (nuclear associates mitotic apparatus), heat shock proteins, redox regulators. The blasts was significantly related presence complex karyotype anomalies. Conditional expression mouse myeloid cell line resulted induction aneuploidy, cycle arrest G(2)-M phases, apoptosis. These results demonstrate potential proteome background-matched obtained fresh clinical specimens provide insight into mechanism leukemogenesis.
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