作者: Iman A. Sylvain , Rachel I. Adams , John W. Taylor
DOI: 10.1371/JOURNAL.PONE.0213355
关键词: Fungal Diversity 、 Toxicology 、 Amplicon sequencing 、 Mold 、 Public housing 、 Fungal biomass 、 Significant difference 、 Fungal genetics 、 Geography 、 Health outcomes
摘要: Water-damaged housing has been associated with a number of negative health outcomes, principally respiratory disease and asthma. Much what we know about fungi water-damaged buildings come from culture-based immunochemical methods. Few studies have used high-throughput sequencing technologies to assess the impact water-damage on microbial communities in residential buildings. In this study amplicon quantitative-PCR evaluate fungal surfaces airborne dust multiple units condemned public project located San Francisco Bay Area. We recruited 21 households participate characterized their apartments as either unit visible mold or no mold. sampled settled over month-long time period outdoors, mold, additionally colonies bathrooms, kitchens, bedrooms, living rooms. found that biomass was greater outdoors compared indoors, but there significant difference Interestingly, diversity reduced outdoors. Units harbored distinct had abundance taxa within classes Eurotiomycetes, Saccharomycetes, Wallemiomycetes. Colonies collected were dominated by two Cladosporium species, C. sphaerospermum C halotolerans. This demonstrates indoors can be useful strategy for distinguishing exposures homes nonvisible growth, may provide means identifying water damaged housing.