Thermoluminescence as a probe of Photosystem II photochemistry. The origin of the flash-induced glow peaks

作者: A.W. Rutherford , A.R. Crofts , Y. Inoue

DOI: 10.1016/0005-2728(82)90061-5

关键词: LuminescencePhotosynthesisFlash (photography)RecombinationPhotosystem IIPhotochemistryPlastoquinoneDCMUChemistryThermoluminescence

摘要: A single flash given at − 15°C to chloroplasts results in charge separation Photosystem II form a stable state which, upon warming, recombines giving rise luminescence. This recombination occurs 25°C untreated but is shifted 0°C the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea or weak concentrations reducing agent. The luminescence attributed S2Q−A while that S2QAQ−B (and S3QAQ−B further illumination). identification thermoluminescence based following experimental evidence: (1) illumination methyl viologen with 710 nm light before and after has no effect on extent temperature thermoluminescence. taken as evidence plastoquinone pool not involved reaction. (2) Calculations expected number flashes, assuming are thermoluminescent reactants, give good fit results. (3) continuous 77 K (i.e., donation from cytochrome b-559 QA thence QB Q−B) predictable changes flash-induced

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