Partition studies on nucleic acids

摘要: Abstract 1. Partition of nucleic acids in liquid two-phase systems composed two polymers, dextran and polyethylene glycol, water is studied with respect to the addition different electrolytes, polymer concentration acid conformation. 2. The tested include native heat denatured DNA from calf thymus, polyadenylic acid, polyuridylic polycytidylic polyinosinic acid. 3. depends highly on electrolyte composition, both ionic strength kinds ions. A small shift ratio between cations or anions can thus transfer high-molecular-weight one phase other. This specific for each type 4. partition single-stranded forms compared double triple-stranded defined compositions. It demonstrated that a change accompanied an increase coefficient 100–1000 times. 5. 1:1 mixture results formation three-stranded complex poly (adenylic + 2 inosinic acids) plus