Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process.
作者： Yohei SHIRAI , Hiroshi YOSHITAKE , Mayuko MARUYAMA , Kenji TAKAMORI , Hideoki OGAWA
DOI: 10.1262/JRD.20156
关键词: Human fertilization 、 Sperm 、 Embryo 、 Epididymis 、 Western blot 、 Biology 、 Monoclonal antibody 、 Epitope 、 Antigen 、 Molecular biology
摘要: To investigate molecular effects of anti-sperm autoantibodies on fertilization, we previously established anti-mouse sperm-head auto-monoclonal antibodies (mAbs). Among the mAbs established, one mAb (named Ts4) recognized sugar moiety TEX101, a germ cell-marker glycoprotein. In present study, examined immunoreactivity Ts4 in mouse spermatozoa and fertilized eggs during early embryogenesis to clarify distribution Ts4-reactive antigen fertilization process. Similar TES101 (a specific probe for TEX101), immunopositive staining was observed spermatocytes, spermatids within testis. contrast results obtained with mAb, reacted sperm acrosomal region cauda epididymis. A Western blot analysis epididymal extract revealed that mainly detected two bands between 100 150 kDa, while faintly band corresponding TEX101 at 38 kDa. addition, molecules were growing embryo after fertilization. Since antigen, potentially carbohydrate chain, is only reproduction-related areas such as testis, embryo, it expected have an effect Therefore, additional studies this may elucidate mechanisms underlying reproductive
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sci-hub.se 参考文章(30)
SUSAN J. KIMBER, Changes in cell-surface glycoconjugates during embryonic development demonstrated using lectins and other probes. Biochemical Society Transactions. ,vol. 17, pp. 23- 27 ,(1989) , 10.1042/BST0170023
S. Sharma, T. C. Schulz, T. C. Schulz, Peter David Rathjen, Joy R. Rathjen, T. A. Pelton, S. Sharma, Transient pluripotent cell populations during primitive ectoderm formation: correlation of in vivo and in vitro pluripotent cell development. Journal of Cell Science. ,vol. 115, pp. 329- 339 ,(2002) , 10.1242/JCS.115.2.329
H HAMADA, Differential expression of lectin receptors in germ layers of the mouse egg cylinder and teratocarcinomas*1 Experimental Cell Research. ,vol. 144, pp. 489- 495 ,(1983) , 10.1016/0014-4827(83)90430-5
Hiroshi Takei, Tadashi Watanabe, Yukiko Sato, Takao Yamashita, Kazuhiko Sudo, Motomu Kuroki, Junichi Chihara, Fujiro Sendo, Kazuhisa Ohtake, A Monoclonal Antibody Modulates Neutrophil Adherence While Enhancing Cell Motility Microbiology and Immunology. ,vol. 41, pp. 67- 72 ,(1997) , 10.1111/J.1348-0421.1997.TB01174.X
Monika Fijak, Andreas Meinhardt, The testis in immune privilege Immunological Reviews. ,vol. 213, pp. 66- 81 ,(2006) , 10.1111/J.1600-065X.2006.00438.X
Pascal M Lanctot, Fred H Gage, Ajit P Varki, None, The Glycans of Stem Cells Current Opinion in Chemical Biology. ,vol. 11, pp. 373- 380 ,(2007) , 10.1016/J.CBPA.2007.05.032
Hiroshi Yoshitake, Yohei Shirai, Yasuhiro Mochizuki, Hiroko Iwanari, Hiroshi Tsubamoto, Koji Koyama, Kenji Takamori, Hideoki Ogawa, Akiko Hasegawa, Tatsuhiko Kodama, Takao Hamakubo, Yoshihiko Araki, Molecular diversity of TEX101, a marker glycoprotein for germ cells monitored with monoclonal antibodies: variety of the molecular characteristics according to subcellular localization within the mouse testis. Journal of Reproductive Immunology. ,vol. 79, pp. 1- 11 ,(2008) , 10.1016/J.JRI.2008.05.001
Peter Cartwright, Cameron McLean, Allan Sheppard, Duane Rivett, Karen Jones, Stephen Dalton, LIF/STAT3 controls ES cell self-renewal and pluripotency by a Myc-dependent mechanism. Development. ,vol. 132, pp. 885- 896 ,(2005) , 10.1242/DEV.01670
A. Fein, Lectin Receptors on Preimplantation Mouse Embryos Gynecologic and Obstetric Investigation. ,vol. 24, pp. 101- 106 ,(1987) , 10.1159/000298787
Anthony L. Tarentino, Caroline M. Gomez, Thomas H. Plummer, Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F Biochemistry. ,vol. 24, pp. 4665- 4671 ,(1985) , 10.1021/BI00338A028