Increased submaximal insulin-stimulated glucose uptake in mouse skeletal muscle after treadmill exercise
作者： Taku Hamada , Edward B. Arias , Gregory D. Cartee
DOI: 10.1152/JAPPLPHYSIOL.00416.2006
关键词: Insulin 、 Carbohydrate metabolism 、 AMP-activated protein kinase 、 Glucose transporter 、 AMPK 、 Glucose uptake 、 Exertion 、 Endocrinology 、 Internal medicine 、 Glycogen 、 Biology
摘要: with [ 3 H]-2-deoxyglucose without or insulin (60 U/ml) to measure glucose uptake. Insulin-stimulated uptake for paired muscles was calculated by subtracting from insulin. Muscles other mice were assessed glycogen and AMPK Thr 172 phosphorylation. Exercised vs. sedentary had decreased in epitrochlearis (48%, P0.001), soleus (51%, P 0.001), EDL (41%, 0.01) increased phosphorylation (P 0.05) (1.7-fold), (2.0-fold), (1.4-fold). Insulin-independent 30 min postexercise the (1.2-fold, (1.4-fold, 0.05), (1.3-fold, 0.01). Insulinstimulated 85 after exercise (sedentary: 0.266 0.045 molg 1 15 ; exercised: 0.414 0.051) 0.102 0.049; 0.347 0.098) but not EDL. Akt Ser 473 308 insulin-stimulated did differ exercised sedentary. These results demonstrate enhanced submaximal insulinstimulated of suggest that it will be feasible probe mechanism sensitivity using genetically modified mice.
sci-hub.se 参考文章(47)
G. D. Cartee, E. E. Bohn, Growth hormone reduces glucose transport but not GLUT-1 or GLUT-4 in adult and old rats American Journal of Physiology-endocrinology and Metabolism. ,vol. 268, ,(1995) , 10.1152/AJPENDO.1995.268.5.E902
Julius A. Gordon, Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods in Enzymology. ,vol. 201, pp. 477- 482 ,(1991) , 10.1016/0076-6879(91)01043-2
A.G. Douen, T. Ramlal, S. Rastogi, P.J. Bilan, G.D. Cartee, M. Vranic, J.O. Holloszy, A. Klip, Exercise induces recruitment of the “insulin-responsive glucose transporter”. Evidence for distinct intracellular insulin- and exercise-recruitable transporter pools in skeletal muscle. Journal of Biological Chemistry. ,vol. 265, pp. 13427- 13430 ,(1990) , 10.1016/S0021-9258(18)77362-6
E. A. Gulve, G. D. Cartee, J. R. Zierath, V. M. Corpus, J. O. Holloszy, Reversal of enhanced muscle glucose transport after exercise: roles of insulin and glucose. American Journal of Physiology-endocrinology and Metabolism. ,vol. 259, ,(1990) , 10.1152/AJPENDO.1990.259.5.E685
D. A. Young, H. Wallberg-Henriksson, M. D. Sleeper, J. O. Holloszy, Reversal of the exercise-induced increase in muscle permeability to glucose American Journal of Physiology-endocrinology and Metabolism. ,vol. 253, ,(1987) , 10.1152/AJPENDO.1987.253.4.E331
G. D. Cartee, D. A. Young, M. D. Sleeper, J. Zierath, H. Wallberg-Henriksson, J. O. Holloszy, Prolonged increase in insulin-stimulated glucose transport in muscle after exercise American Journal of Physiology-endocrinology and Metabolism. ,vol. 256, pp. 494- 499 ,(1989) , 10.1152/AJPENDO.1989.256.4.E494
G. D. Cartee, A. G. Douen, T. Ramlal, A. Klip, J. O. Holloszy, Stimulation of glucose transport in skeletal muscle by hypoxia Journal of Applied Physiology. ,vol. 70, pp. 1593- 1600 ,(1991) , 10.1152/JAPPL.1991.70.4.1593
L. P. Garetto, E. A. Richter, M. N. Goodman, N. B. Ruderman, Enhanced muscle glucose metabolism after exercise in the rat: the two phases. American Journal of Physiology-endocrinology and Metabolism. ,vol. 246, ,(1984) , 10.1152/AJPENDO.1984.246.6.E471
Patrick T. Fueger, Jane Shearer, Tess M. Krueger, Kelly A. Posey, Deanna P. Bracy, Sami Heikkinen, Markku Laakso, Jeffrey N. Rottman, David H. Wasserman, Hexokinase II protein content is a determinant of exercise endurance capacity in the mouse. The Journal of Physiology. ,vol. 566, pp. 533- 541 ,(2005) , 10.1113/JPHYSIOL.2005.085043
H. Wallberg-Henriksson, S. H. Constable, D. A. Young, J. O. Holloszy, Glucose transport into rat skeletal muscle: interaction between exercise and insulin Journal of Applied Physiology. ,vol. 65, pp. 909- 913 ,(1988) , 10.1152/JAPPL.1988.65.2.909