Bidirectional effects of methanol extract of Wei-Chang-An pill on gastrointestinal transit and the spasmolytic activity on isolated rat jejunum.

摘要: Abstract Ethnopharmacological relevance Wei-Chang-An pill (WCA pill), a traditional Chinese medicine, has been used for treating various gastrointestinal diseases several decades. Despite the popular medicinal use of WCA pill, less data was available to its activity and mechanism in disorders. To examine effects methanol extract (ME) on tract so as assess some possible mechanisms involved clinical treatment. Materials methods ME studied transit vivo including gastric emptying small intestinal motility normal neostigmine-induced mice, well isolated tissue preparations rat jejunum vitro . Results In , decreased increased after administration mice. However, accelerated ranging from 0.01 0.8 mg/mL reduced it at concentration 1.6 3.2 mg/mL, while inhibited throughout concentrations caused inhibitory effect spontaneous contraction rat-isolated dose-dependent manner 6 mg/mL also relaxed acetylcholine chloride (Ach, 10 −6  M)-induced K + (60 mM)-induced contractions. shifted Ca 2+ concentration–response curves right, similar that by verapamil (0.025 mM). Conclusions These results indicated might play bidirectional role modulation are probably mediated through calcium influx muscarinic receptors, which provides pharmacological basis