Genome-wide introgression from a bread wheat × Lophopyrum elongatum amphiploid into wheat

摘要: We introgressed wheatgrass germplasm from the octoploid amphiploid Triticum aestivum× Lophopyrum elongatum into wheat by manipulating Ph1 gene and discovered characterized 130 introgression lines harboring single or, in various combinations, complete recombined L. chromosomes. Diploid Lophopyrum (genomes EE) possesses valuable traits for genetics breeding. evaluated several strategies of this wheat. To detect it, we developed validated multiplexed sets Sequenom MassARRAY nucleotide polymorphism (SNP) markers, which differentiated disomic monosomic chromosomes identified (ILs), harbored 108 89 Of latter, 59 were one or more crossovers 30 involved centromeric (Robertsonian) translocations telocentric. identify substituted with chromosomes, genotyped ILs 90-K Infinium SNP array. found that most probes correctly detected their targets genome showed some SNPs are ancient had originated prior to divergence lineages. ILs, 52% homozygous deletion thus staged be further. failed 4/5 reciprocal translocation has been reported Thinopyrum bessarabicum other Triticeae genomes.