Retinal expression of gamma-crystallins in the mouse.

摘要: PURPOSE: High levels of expression a form gamma-crystallin mRNA in mouse retina have been identified. Because the six murine gamma-crystallins generally regarded as specific to lens, these crystallins at and protein were evaluated more detail. METHODS: Expression gammaE/F-crystallin was examined by northern blot analysis reverse transcription-polymerase chain reaction (RT-PCR) applied retinal lens total RNAs. For gammaA-D-crystallin mRNAs, multiplex RT-PCR used on cDNAs. The detection performed using an antibody bovine gamma-crystallins, extracts immunoblot cryostat sections ocular tissues immunofluorescence studies. RESULTS: By RT-PCR, we confirmed both gammaE-and gammaF-crystallin well all four (gammaA-gammaD) remaining level retina. Gamma-crystallin proteins also detectable analysis, although lower than lens. immunocytochemistry, localized particularly inner retina, outer plexiform layer, photoreceptors during postnatal development. CONCLUSIONS: Our findings indicate that none major crystallin classes is uniquely expressed developing suggests role analogous anti-stress properties established for small heat-shock alphaB-crystallin, perhaps response varying exposure light.