Reductive Pentose Phosphate Cycle in Nitrosocystis oceanus1

摘要: Campbell, Ann E. (Woods Hole Oceanographic Institution, Woods Hole, Mass.), Johan A. Hellebust, and Stanley W. Watson. Reductive pentose phosphate cycle in Nitrosocystis oceanus. J. Bacteriol. 91:1178–1185. 1966.—Assays cell-free extracts of oceanus, a marine chemoautotrophic bacterium, have demonstrated the presence all enzymes reductive cycle, with activities high enough to account for normal growth rate cells. Studies on ribulosediphosphate carboxylase activity these showed that it is inhibited by MgCl2 (30% at 0.01 m), MnCl2 (70% NaCl KCl (100% 0.5 m, 63% 0.2 sulfate (35% m); phosphate, glutathione, ethylenediaminetetraacetic acid had no effect. The bacterial enzyme differs from spinach respect its affinity bicarbonate pH optimum. Whole cells were incubated C14O2, acid-soluble fraction was analyzed paper chromatography autoradiography. Phosphoglyceric sugar phosphates earliest labeled compounds; several amino acids organic also labeled. It concluded N. oceanus incorporates CO2 primarily via cycle.