The Antibody Repertoire of Hybrid Cell Lines Obtained by Fusion of X63-AG8 Myeloma Cells with Mitogen-Activated B-Cell Blasts
作者： J. Andersson , F. Melchers
DOI: 10.1007/978-3-642-67448-8_19
关键词: In vivo 、 Virus quantification 、 Spleen 、 Cell culture 、 Biology 、 Mitogen-activated protein kinase 、 Fusion 、 B cell 、 Molecular biology 、 Virology 、 Antibody Repertoire
摘要: Fusion of myeloma cells with antibody-secreting B-cells has been achieved in vivo antigen-activated spleen (1). Such fusions appeared to occur at a stage differentiation similar that the line, i.e. antigen-activated, B-cell blasts. This was concluded from high incidence hybrid cell lines and by absence T-cell-B-cell hybrids these (2).
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