DNA fingerprinting of the NCI-60 cell line panel.

摘要: The National Cancer Institute's NCI-60 cell line panel, the most extensively characterized set of cells in existence and a public resource, is frequently used as screening tool for drug discovery. Because many laboratories around world rely on data from cells, confirmation their genetic identities represents an essential step validating results them. Given consequences contamination or misidentification, quality control measures should routinely include DNA fingerprinting. We have, therefore, standard microsatellite short tandem repeats to profile NCI-60, resulting fingerprints are provided here reference. Consistent with previous reports, suggest that several lines have common origins: melanoma MDA-MB-435, MDA-N, M14; central nervous system U251 SNB-19; ovarian OVCAR-8 OVCAR-8/ADR (also called NCI/ADR); prostate DU-145, DU-145 (ATCC), RC0.1. Those also show ability connect two same origin not affected by stable transfection development multidrug resistance. As expected, were able distinguish different tissues-of-origin. serve principally barcodes.